Ju Seok Lee
Postdoctoral Associate
Department of Biological and Agricultural Engineering
University of Arkansas
Fayetteville, AR 72701

EDUCATION

2009 Ph.D., Cell and Molecular Biology, University of Arkansas, USA
Title: A Nucleic Acid-Based Biological Memory with In Vitro Adaptive Learning and Associative Recall
2000 M.S., Agronomy, Seoul National University, Korea
Title: Relationship between genetic distance measures and F1 heterosis in soybean
1998 B.S., Agronomy, Seoul National University, Korea
POSITIONS and EMPLOYMENTS

2002 – 2009 Research assistant at the Bio/Nano Technology Laboratory at University of Arkansas
2009 – present Postdoctoral associate at the Bio/Nano Technology Laboratory at University of Arkansas
OTHER EXPERIENCE and PROFESSIONAL MEMBERSHIPS

2009 – present Ad-hoc journal article review: Journal of Biological Engineering; Nanoscale Research Journal
2004 – present Member, Alpha Epsilon
2005 – present Member, American Chemical Society
2005 – present Member, American Association for the Advancement of Science
2005 – present Member, Institute of Biological Engineering

 
AVAILABLE TECHNIQUES and TOOLS for RESEARCH

1. DNA techniques
a. Microarray printing, hybridization, image scanning and its statistical analysis
b. Agarose gel electrophoresis and its analysis
c. Urea-TBE polyacrylamide gel electrophoresis (PAGE) and its analysis
d. Denaturing gradient gel electrophoresis (DGGE) and its analysis
e. DNA amplification using PCR and its analysis
f. DNA analysis using real-time quantitative PCR
g. DNA manipulation using various enzymes, such as ligase or Klenow fragment
h. DNA purification using various commercial kits or manual methods

2. Bacteria, protozoa and mammalian cell lines maintenance and DNA extraction
a. Various bacterial strains (E.coli, B.subtilis, S.aureus and so on)
b. Protozo (C.parvum )
c. Mammalian cell line (HCT-8 human ileocecal colorectal carcinoma cell line)

3. Gene cloning
a. DNA extraction from various organisms (plant or bacteria)
b. Subtractive cloning and library construction
c. Southern and northern blotting using radio-active labeling method and its analysis
d. Electroporation and inserted fragment screening using GFP-gene

4. Programming and computation
a. Simple algorithm developing and programming using Perl
b. JMP for statistical analysis
c. R project for statistical computing
d. Microsoft office program group (Word, Excel, Powerpoint, and Visio)
e. SigmaPlot for scientific data analysis and graphing

5. Image analysis
a. Gel image analysis using Gel Doc™ system (Bio-Rad)
b. AFM (atomic force microscope) scanning and its image analysis

6. Nanoparticle manipulation
a. Single-Walled Carbon Nanotube (SWNT) preparation using acid-sonication
b. Size-defined gold nanoparticle synthesis using surfactant
 
RESEARCH

My primary research interest is in the area of Bio/Nano Technololgy, which is a new emerging, interdisciplinary field of research. During my M.S. and Ph.D degree, I have been trained to work with various field of research, such as nucleic acid technology, DNA computation, gene cloning, nanotechnology and biotechnology. Based on my research experience and knowledge, my research will be focused on developing the novel diagnostic methods and tools, which require the knowledge, both biology and nanotechnology. Also I have a experience with a field works and research for the sampling, and maintaining the plant and microorganisms. I have a strong background of statistics, so I can perform the most of statistical analysis, which is required for the scientific researches and experiments.
PUBLICTIONS
Publications
1. Lee, J.S., Kim, J.-W., Deaton, R. & Chen, J. Genomic Pattern Classifier Based on a Biological Memory with In Vitro Learning and Associative Recall. Biotechnique (in review).
2. Lee, J.S., Song, J.J., Kim, J.-W. & Deaton, R. Assessing detection capacity of Microarray. BMC Bioinformatics (in review).
3. Woo, J.-S., J.S. Lee, B.-K. Ha, H.-S. Lee, and S.-H Lee. 2000. Genetic Diversity Among Soybean Genotypes for Sprout. Journal of Crop Science and Biotechnology. 8(1):136-7
Refereed Conference Proceedings
1. B. Srinivasan, J.S. Lee, J. Hohnbaum, S. Tung, and J.-W. Kim. “Performance Evaluation of a Pneumatic-Based Micromixer for Bioconjugation Reaction,” in Proceedings of 5th IEEE International Conference on Nano/Micro Engieered and Molecular Systems (IEEE-NEMS), Xiamen, China (accepted).
2. J.S. Lee, J.J. Song, R. Deaton, and J.-W. Kim. 2009. “Exploring the Potential of Microarray Technology for Bio/Nano Sensing,” in Proceedings of IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS), Shenzen, China.
3. J.-W. Kim, J.S. Lee, and R. Deaton. 2007. “Non-crosshybridizing oligonucleotide building blocks for accurate and scalable nanostructure assembly,” in Proceedings of IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS), Bangkok, Thailand.
4. J.-W. Kim, R. Deaton, J. Chen, and J.S. Lee. 2006. “Genomic Pattern Classifier Based on a Biological Memory with In Vitro Learning and Associative Recall,” in DNA 12: Preliminary Proceedings of the 12th International Meeting on DNA Computing, C. Mao, T. Yokomori, and B.-T. Zhang (eds.), Seoul, Korea.
5. J. Chen, R. Deaton, M. Garzon, J.-W. Kim, D. H. Wood, H. Bi, D. Carpenter, J. S. Lee, and Y. Z. Wang. 2005. “Sequence complexity of large libraries of DNA oligonucleotides,” in DNA Computing: Preliminary Proceedings of the 11th International Workshop on DNA-Based Computers, N. Pierce and A. Carbone, (eds.), London, Ontario, Canada.
6. R. Pooran, M. Al-Fandi, S. Tung, J.-W. Kim, and J.S. Lee. 2004. “Bacterial flagellar motors as microfluidic actuators,” in Proceedings of ASME International Mechanical Engineering Congress and R&D Expo (IMECE), Anaheim, CA.
Refereed Conference Presentations
1. B. Srinivasan, J.S. Lee, J. Hohnbaum, S. Tung, and J.-W. Kim. Performance Evaluation of a Pneumatic-Based Micromixer for Bioconjugation Reaction. 5th IEEE International Conference on Nano/Micro Engieered and Molecular Systems (IEEE-NEMS), Xiamen, China (accepted).
2. J.S. Lee, J.J. Song, R. Deaton, and J.-W. Kim. 2009. Exploring the Potential of Microarray Technology for Bio/Nano Sensing. IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS), Shenzen, China.
3. J.-W. Kim, J.S. Lee, and R. Deaton. 2007. Non-Crosshybridizing Oligonucleotide Building Blocks for Accurate, Scalable, and High-Rate Nanofabrication. IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS), Bangkok, Thailand.
4. J.-W. Kim, R. Deaton, J. Chen, and J.S. Lee. 2006. Genomic Pattern Classifier Based on a Biological Memory with In Vitro Learning and Associative Recall. DNA 12: 12th International Meeting on DNA Computing, Seoul, Korea.
5. J.-W. Kim, R. Deaton, J.S. Lee, and H.D. Wijesekera. 2006. In Vitro Selection for Large Libraries of Non-Crosshybridizing DNA Oligonucleotides for DNA Computing and DNA-Based Nanotechnology. Institute of Biological Engineering (IBE) Annual Meeting, Tucson, AZ.
6. J.-W. Kim, J.S. Lee, and R. Deaton. 2006. A Biological Memory with In Vitro Learning and Associative Recall as a Genomic Pattern Classifier. Institute of Biological Engineering (IBE) Annual Meeting, Tucson, AZ.
7. J.-W. Kim, R. Deaton, J.S. Lee, and J. Chen. 2005. Thermal Melting and Cot Analyses of Non-Crosshybridizing DNA Oligonucleotides Manufactured In Vitro. Institute of Biological Engineering (IBE) Annual Meeting, Athens, GA.
8. J.-W. Kim, S. Tung, J.S. Lee, N.K. Kotagiri, R. Pooran, and M. Al-Fandi. 2004. A Hybrid Flagellar Motor/MEMS Micro-Pump. 96th AIChE Ann. Meet. (Nanoscale Science and Engineering Session), Austin, TX.
9. J.-W. Kim, S. Tung, R. Deaton, J.S. Lee, and I. Kodikara. 2004. DNA-Directed Self-Assembly of Single-Walled Carbon Nanotubes through Controlled DNA-Nanotube Interfacing. Institute of Biological Engineering (IBE) Annual Meeting, Fayetteville, AR.
10. R. Pooran, S. Tung, J.-W. Kim, A. Malshe, and J.S. Lee. 2004. Performance of a Flagellar Motor Based Pump. Institute of Biological Engineering (IBE) Annual Meeting, Fayetteville, AR (won 1st place in the IBE student poster competition).